• Gram Staining Procedure Pdf Download rescate guegos mundos muriendo

     

    Gram Staining Procedure Pdf Download >>> http://tinyurl.com/y7whkshe

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

    Gram Staining Procedure Pdf Download

     

    Gm+...organisms...are...thought...to...resist...decolorization...byalcohol...or...acetone...because...cell...wall...permeability...is...markedly...decreased...whenit...is...dehydrated...by...these...solvents.Observation:....----Results:....------Flow....chart....Bacterial....smear....preparationHeat....fixationStain....with....cryastal....violet....1minuteWash....with....water....until....crystal....violet....disappearsFlood....with....Iodine....1.5minuteWash....with....water....Footer....MenuBack....To....TopAboutAbout....ScribdPressOur....blogJoin....our....team!Contact....UsJoin....todayInvite....FriendsGiftsLegalTermsPrivacyCopyrightSupportHelp..../....FAQAccessibilityPurchase....helpAdChoicesPublishersSocial....MediaCopyright........2017....Scribd....IncGram..Positive:..Blue/Purple..Color..Gram..Negative:..Red..Color..Flood...the...grams...iodine...for...1...minute...and...wash...with...waterGram..Staining..Animation118Take...a...clean,...grease...free...slide

     

    This..stain..differentiatestwo..main..categories..of..bacteria..according..to..the..structure..of..the..cell..wall.Bacteria..with..a..predominantly..peptidoglycan..cell..wall..stain..blue/purple..withthis..technique..and..since..they..retain..the..primary..stain..are..called..Gram..positive(Gram..+)When....they....are....exposed....to....alcohol,decolorizer....dissolves....the....lipids....in....the....cell....walls,....which....allows....the....crystal....violet-iodine....complex....to....leach....out....of....the....cellsOne...function...of...fixation...is...to...secure...(fix)...the...cells...to...the...slide.Examples...of...Gram...positive...Bacteria...CorynebacteriumLactobacillusBacillusStreptococcusStaphylococcusClostridium......Examples...of...Gram...negative...Bacteria...Escherichia...coliKlebsiellaSalmonellaShigellaPseudomonasCampylobacterVibrio...choleraeYersinia......Principle...Both...Gram-positive...(Gm+)...and...Gram-negative...(Gm)...organisms...form...acomplex...of...crystal...violet...and...iodine...within...the...bacterial...cell...during...the...Gram-......staining...procedureWhen..the..bacteria..is..stained..with..primary..stain..Crystal..Violet..and..fixed..by..the..mordant,..some..of..the..bacteria..are..able..to..retain..the..primary..stain..and..some..are..decolorized..by..alcoholDownload...Animation...from...Below:Then..when..again..stained..with..safranin,..they..take..the..stain..and..appears..red..in..colorIntroduction:........The....Gram....stain....devised....by....Christian....Gram....in....1882....has....become....one....of....themost....important....diagnostic....procedures....in....microbiologyAllow..to..air-dry.2.Carefully..fixate..the..cells..by..moving..the..slide..into..a..flame..(bacteriaupwards)A....Gram....stain....is....usually....performed....on....a....smear....preparation....that....hasbeen....heat....fixedIn..case..of..gram..negative..bacteria,..cell..wall..also..takes..up..the..CV-Iodine..complex..but..due..to..the..thin..layer..of..peptidoglycan..and..thick..outer..layer..which..is..formed..of..lipids,..CV-Iodine..complex..gets..washed..offCells...with...only...a...minor...peptidoglycan...component...and...possessing...alipopolysaccharide...cell...wall...give...up...the...primary...stain...during...destaining...andtherefore...exhibit...the...red...counter...stainAir..dry,..Blot..dry..and..Observe..under..Microscope...410 87c6bb4a5b


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